Article

Dissection of the cellular function of the ZBED6 transcription factor in mouse myoblast cells using gene editing, RNAseq and proteomics

2019-02-05

Abstract excerpt

<h4>SUMMARY</h4> The transcription factor ZBED6 acts as a repressor of Igf2 and affects directly or indirectly the transcriptional regulation of thousands of genes. Here, we use gene editing in mouse C2C12 myoblasts and show that ZBED6 regulates Igf2 exclusively through its binding site 5′-GGCTCG-3′ in intron 1 of Igf2 . Deletion of this motif ( Igf2 ΔGGCT ) or complete ablation of Zbed6 leads to ~20-fold up...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: d40a7b7e-d469-5296-9cff-fd2982afdf6e
DOI: 10.1101/540914

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Dissection of the cellular function of the ZBED6 transcription factor in mouse myoblast cells using gene editing, RNAseq and proteomicsDOI 10.1101/540914Read

Growth retardation in a mouse model of Kabuki syndrome 2 bears mechanistic similarities to Kabuki syndrome 1Semantic proximity Read

SIX1 Reprograms Myogenic Transcription Factors to Maintain the Rhabdomyosarcoma undifferentiated stateSemantic proximity Read

DiPRO1 dependent transcriptional and epigenetic regulation distinctly controls the fate of muscle and mesenchymal cancer cellsSemantic proximity Read

Efficient Editing of the ZBED6-Binding Site in Intron 3 of IGF2 in a Bovine Model Using the CRISPR/Cas9 System.Semantic proximity Read

The ZBED6-IGF2 axis has a major effect on growth of skeletal muscle and internal organs in placental mammals.Semantic proximity Read

SIX1 reprograms myogenic transcription factors to maintain the rhabdomyosarcoma undifferentiated stateSemantic proximity Read

Gene co-expression network analysis provides novel insights into myostatin regulation at three different mouse developmental timepoints.Semantic proximity Read

TEAD transcription factors are required for normal primary myoblast differentiation in vitro and muscle regeneration in vivo.Semantic proximity Read

Identification of MAZ as a novel transcription factor regulating erythropoiesisSemantic proximity Read

Ccndbp1 is a new positive regulator of skeletal myogenesis.Semantic proximity Read

DNA methylation of Ad4BP/SF-1 suppresses Cyp11a1 and StAR transcripts in C2C12 myoblastsSemantic proximity Read

Posttranslational modifications of the DUX4 protein impact toxic functionSemantic proximity Read