Article
Efficient Editing of the ZBED6-Binding Site in Intron 3 of IGF2 in a Bovine Model Using the CRISPR/Cas9 System.
Genes - 24 Jun 2022
Zou Huiying, Yu Dawei, Yao Shun, Ding Fangrong, Li Junliang, Li Ling, Li Xue, Zhao Shanjiang, Pang Yunwei, Hao Haisheng, Du Weihua, Zhao Xueming, Dai Yunping, Zhu Huabin
Abstract excerpt
BACKGROUND: Insulin-like growth factor 2 is a growth-promoting factor that plays an important role in the growth and development of mammals. A nucleotide substitution in intron 3 of IGF2-which disrupts the ZBED6-binding site-affects muscle mass, organ size, and fat deposition in pigs. The ZBED6-binding site is also conserved in cattle. METHODS: In the present study, we introduced mutations in the ZBED6-binding...
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