Article

A simple reverse genetics method to generate recombinant coronaviruses 

2021-03-09

Abstract excerpt

<title>Abstract</title> <p>Engineering recombinant viruses is capital for deciphering the biology of emerging viral pathogens such as the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, the large size of coronaviruses genome makes reverse genetics methods challenging.Here we describe a simple method based on “infectious subgenomic amplicons” (ISA) technology to generate recombinant infectio...

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Literature Corpus work: d0a77823-19a1-5564-859c-02662add5f3e
DOI: 10.21203/rs.3.rs-59766/v3

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