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Establishment of a Reverse Genetics System for SARS-CoV-2 Using Circular Polymerase Extension Reaction

2020-10-23

Abstract excerpt

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been identified as the causative agent of coronavirus disease 2019 (COVID-19). While the development of specific treatments and a vaccine is urgently needed, functional analyses of SARS-CoV-2 have been limited by the lack of convenient mutagenesis methods. In this study, we established a PCR-based, bacterium-free method to generate SARS-CoV-2 infecti...

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Literature Corpus work
dcd011ba-bc71-5314-adc8-2165de366a38
DOI
10.2139/ssrn.3714847
Open publication

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Establishment of a Reverse Genetics System for SARS-CoV-2 Using Circular Polymerase Extension ReactionDOI 10.2139/ssrn.3714847
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