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Establishment of a reverse genetics system for SARS-CoV-2 using circular polymerase extension reaction

2020-09-23

Abstract excerpt

<h4>Summary</h4> Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been identified as the causative agent of coronavirus disease 2019 (COVID-19). While the development of specific treatments and a vaccine is urgently needed, functional analyses of SARS-CoV-2 have been limited by the lack of convenient mutagenesis methods. In this study, we established a PCR-based, bacterium-free method to generate...

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Literature Corpus work
d84082c2-9814-55b3-ac4f-37f136af5373
DOI
10.1101/2020.09.23.309849
Open publication

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Establishment of a reverse genetics system for SARS-CoV-2 using circular polymerase extension reactionDOI 10.1101/2020.09.23.309849
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