Article
A simple reverse genetics method to generate recombinant coronaviruses
3 Mar 2022
Abstract excerpt
Engineering recombinant viruses is a pre-eminent tool for deciphering the biology of emerging viral pathogens such as the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, the large size of coronavirus genomes renders the current reverse genetics methods challenging. Here, we describe a simple method based on "infectious subgenomic amplicons" (ISA) technology to generate recombinant...
Topics
Join the communities discussing this publication.