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A simple reverse genetics method to generate recombinant coronaviruses

3 Mar 2022

Abstract excerpt

Engineering recombinant viruses is a pre-eminent tool for deciphering the biology of emerging viral pathogens such as the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, the large size of coronavirus genomes renders the current reverse genetics methods challenging. Here, we describe a simple method based on "infectious subgenomic amplicons" (ISA) technology to generate recombinant...

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PMID: 35239997
DOI: 10.15252/embr.202153820

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