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Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platform

11 Aug 2023

Abstract excerpt

Reverse genetic systems enable the engineering of RNA virus genomes and are instrumental in studying RNA virus biology. With the recent outbreak of the coronavirus disease 2019 pandemic, already established methods were challenged by the large genome of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Herein we present an elaborated strategy for the rapid and straightforward rescue of recombinant...

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PMID: 37988285
DOI: 10.7554/elife.89035

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