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Application of versatile reverse genetics system for feline coronavirus

10 Mar 2025

Abstract excerpt

Feline infectious peritonitis (FIP) is a fatal disease caused by feline coronavirus (FCoV). Although multiple gene mutations in FCoV likely account for FIP pathogenesis, molecular studies for FCoV have been limited due to the lack of a suitable reverse genetics system. In the present study, we established a rapid PCR-based system to generate recombinant FCoV using the circular polymerase extension reaction (CPER)...

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PMID: 40062768
DOI: 10.1128/spectrum.02692-24

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