Article

Resolving the complex CES1 genomic locus with Cas9-directed targeted long-read sequencing

2026-08-22

Abstract excerpt

Abstract Carboxylesterase 1 (CES1) is a major hepatic enzyme involved in the metabolism of many ester-containing drugs and endogenous lipids. However, genomic analysis of the CES1 locus is challenging due to highly homologous regions that confound short-read sequencing. To overcome these limitations, we applied an optimized, PCR-free Oxford Nanopore Cas9-directed targeted sequencing (nCATS) approach to enrich and...

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Literature Corpus work: cb1d9dc1-6b94-5624-8cc6-4ce5420af9f3
DOI: 10.1038/s41525-026-00604-w

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