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Article

CRISPR-Act3.0 for highly efficient multiplexed gene activation in plants.

Nature plants - 1 Jul 2021

Pan Changtian, Wu Xincheng, Markel Kasey, Malzahn Aimee A, Kundagrami Neil, Sretenovic Simon, Zhang Yingxiao, Cheng Yanhao, Shih Patrick M, Qi Yiping

Abstract excerpt

RNA-guided CRISPR activation (CRISPRa) systems have been developed in plants. However, the simultaneous activation of multiple genes remains challenging. Here, we develop a highly robust CRISPRa system working in rice, Arabidopsis and tomato, CRISPR-Act3.0, through systematically exploring different effector recruitment strategies and various transcription activators based on deactivated Streptococcus pyogenes...

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Identifiers

PMID: 34168320
DOI: 10.1038/s41477-021-00953-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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