Article
Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.
Proceedings of the National Academy of Sciences of the United States of America - 17 Mar 2015
Xie Kabin, Minkenberg Bastian, Yang Yinong
Abstract excerpt
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 nuclease (Cas9) system is being harnessed as a powerful tool for genome engineering in basic research, molecular therapy, and crop improvement. This system uses a small guide RNA (gRNA) to direct Cas...
Topics
- Base Sequence
- CRISPR-Cas Systems
- Genes, Plant
- Genetic Engineering
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Oryza
- Plants, Genetically Modified
- Protoplasts
- RNA Editing
- RNA Processing, Post-Transcriptional
- RNA, Guide, CRISPR-Cas Systems
- RNA, Transfer