Article
Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.
Proceedings of the National Academy of Sciences of the United States of America - 17 Mar 2015
Xie Kabin, Minkenberg Bastian, Yang Yinong
Abstract excerpt
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 nuclease (Cas9) system is being harnessed as a powerful tool for genome engineering in basic research, molecular therapy, and crop improvement. This system uses a small guide RNA (gRNA) to direct Cas9 endonuclease to a specific DNA site; thus, its targeting capability is largely constrained by the gRNA-expressing...
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