Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice.

Communications biology - 23 Jan 2020

Ali Zahir, Shami Ashwag, Sedeek Khalid, Kamel Radwa, Alhabsi Abdulrahman, Tehseen Muhammad, Hassan Norhan, Butt Haroon, Kababji Ahad, Hamdan Samir M, Mahfouz Magdy M

Abstract excerpt

Precise genome editing by systems such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) requires high-efficiency homology-directed repair (HDR). Different technologies have been developed to improve HDR but with limited success. Here, we generated a fusion between the Cas9 endonuclease and the Agrobacterium VirD2 relaxase (Cas9-VirD2). This chimeric protein...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to ATP-Binding Cassette Transporters
Share to CRISPR-Associated Protein 9

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Endodeoxyribonucleases

Share to Gene Editing

Share to Genetic Engineering

Share to Herbicide Resistance

Share to Recombinational DNA Repair

Source

Open in PubMed Open full text

Identifiers

PMID: 31974493
DOI: 10.1038/s42003-020-0768-9
PMCID: PMC6978410

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice.PMID 31974493Read ↗

Efficient protein tagging and <i>cis</i> -regulatory element engineering via precise and directional oligonucleotide-based targeted insertion in plantsSemantic proximity Read ↗

Application of CRISPR/Cas9 Technology in Rice Germplasm Innovation and Genetic ImprovementSemantic proximity Read ↗

Cas9-PE: a robust multiplex gene editing tool for simultaneous precise editing and site-specific random mutation in rice.Semantic proximity Read ↗

Using CRISPR-Cas9 to generate semi-dwarf rice lines in elite landraces.Semantic proximity Read ↗

A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants.Semantic proximity Read ↗

CRISPR-Act3.0 for highly efficient multiplexed gene activation in plants.Semantic proximity Read ↗

Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems.Semantic proximity Read ↗

Characteristics of Genome Editing Mutations in Cereal Crops.Semantic proximity Read ↗

Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice.Semantic proximity Read ↗

PAM‐relaxed and temperature‐tolerant CRISPR‐Mb3Cas12a single transcript unit systems for efficient singular and multiplexed genome editing in rice, maize, and tomatoSemantic proximity Read ↗

Generation and Transcriptome Profiling of Slr1-d7 and Slr1-d8 Mutant Lines with a New Semi-Dominant Dwarf Allele of SLR1 Using the CRISPR/Cas9 System in Rice.Semantic proximity Read ↗

Addition of the T5 exonuclease increases the prime editing efficiency in plants.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.