Article

Harnessing secretory pathway differences between HEK293 and CHO to rescue production of difficult to express proteins

2021-08-17

Abstract excerpt

<h4>Summary</h4> Biologics represent the fastest growing group of therapeutics, but many advanced recombinant protein moieties remain difficult to produce. Here, we identify bottlenecks limiting expression of recombinant human proteins through a systems biology analysis of the transcriptomes of CHO and HEK293 during recombinant overexpression. Surprisingly, one third of the challenging human proteins displayed im...

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Literature Corpus work: c54cab04-de14-587e-8db7-8420a9a2aa62
DOI: 10.1101/2021.08.16.455786

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