Article

Transcriptome analysis of EPO and GFP HEK293 Cell-lines Reveal Shifts in Energy and ER Capacity Support Improved Erythropoietin Production in HEK293F Cells

2020-09-16

Abstract excerpt

<h4>Summary</h4> Higher eukaryotic cell lines like HEK293 are the preferred hosts for production of therapeutic proteins requiring human post translational processing. However, recombinant protein production can result in severe stress on the cellular machinery, resulting in limited titre and product quality. To investigate the cellular and metabolic characteristics associated with these limitations, we compared...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: b8e8f718-e653-530a-bb0b-897dc88493c0
DOI: 10.1101/2020.09.16.299966

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Transcriptome analysis of EPO and GFP HEK293 Cell-lines Reveal Shifts in Energy and ER Capacity Support Improved Erythropoietin Production in HEK293F CellsDOI 10.1101/2020.09.16.299966Read

Harnessing secretory pathway differences between HEK293 and CHO to rescue production of difficult to express proteinsSemantic proximity Read

Deciphering the determinants of recombinant protein yield across the human secretomeSemantic proximity Read

DTT-Induced Transcriptome Profiling Reveals Divergent ER Proteostasis and Stress-Adaptation Programs in CHO-S and Apoptosis-Resistant CHO-4BGD Producer Cell LinesSemantic proximity Read

HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral VectorsSemantic proximity Read

Human cellular model systems of β-thalassemia enable in-depth analysis of disease phenotypeSemantic proximity Read

Phenotypes and Multi-omics Reveal Changes and Molecular Mechanism of Suspension Adaptation of HEK293 Cells: Structural Remodelling, Metabolic Reconstruction and Stress ResistanceSemantic proximity Read

Phenotypic and proteomic characterization of the human erythroid progenitor continuum reveal dynamic changes in cell cycle and in metabolic pathwaysSemantic proximity Read

Engineering of Chinese hamster ovary cells for co-overexpressing MYC and XBP1s increased cell proliferation and recombinant EPO productionSemantic proximity Read

A scalable strategy for high-throughput GFP tagging of endogenous human proteinsSemantic proximity Read

Spatiotemporal Proteome Remodeling Directs Human Hematopoietic Stem Cells via a Metabolic-Cytoskeletal AxisSemantic proximity Read

Integrative proteomics reveals principles of dynamic phosphosignaling networks in human erythropoiesisSemantic proximity Read

Efficient cell-free translation from diverse human cell typesSemantic proximity Read