Article

Unlocking HDR-mediated Nucleotide Editing by identifying high-efficiency target sites using machine learning

2018-11-07

Abstract excerpt

Editing individual nucleotides is a crucial component for validating genomic disease association. It currently is hampered by CRISPR-Cas-mediated “base editing” being limited to certain nucleotide changes, and only achievable within a small window around CRISPR-Cas target sites. The more versatile alternative, HDR (homology directed repair), has a 4-fold lower efficiency with known optimization factors being large...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: c5082a03-79d0-5cd8-a5e7-746a685d6041
DOI: 10.1101/464610

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Unlocking HDR-mediated Nucleotide Editing by identifying high-efficiency target sites using machine learningDOI 10.1101/464610Read

Unlocking HDR-mediated nucleotide editing by identifying high-efficiency target sites using machine learningSemantic proximity Read

Predicting base editing outcomes using position-specific sequence determinantsSemantic proximity Read

Predicting Mutations Generated by Cas9, Base Editing, and Prime Editing in Mammalian Cells.Semantic proximity Read

Mechanistic machine learning enables interpretable and generalizable prediction of prime editing outcomesSemantic proximity Read

Predicting base editing outcomes using position-specific sequence determinantsSemantic proximity Read

Comprehensive evaluation and prediction of editing outcomes for near-PAMless adenine and cytosine base editorsSemantic proximity Read

The interplay of DNA repair context with target sequence predictably biases Cas9-generated mutations.Semantic proximity Read

<i>beditor</i> : A computational workflow for designing libraries of guide RNAs for CRISPR-mediated base editingSemantic proximity Read

Rapid customization of base editors via machine learning-powered combinatorial mutagenesis.Semantic proximity Read

Compact zinc finger base editors that edit mitochondrial or nuclear DNA in vitro and in vivoSemantic proximity Read

Pythia: Non-random DNA repair allows predictable CRISPR/Cas9 integration and gene editingSemantic proximity Read

MMEJ-based Precision Gene Editing for applications in Gene Therapy and Functional GenomicsSemantic proximity Read