Article

An orthogonal Cro/O <sub>R</sub> 3 binary system for targeted gene expression

2025-12-25

Abstract excerpt

Precise spatiotemporal control of gene expression is essential for dissecting complex biological systems. Here, we introduce a phage-derived Cro/O R 3 binary system for transcriptional activation that functions robustly in zebrafish and human cells. A Cro repressor fused to the VP16 activation domain efficiently drives expression of O R 3 operator-linked genes, and its activity can be targeted to specific cell t...

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Literature Corpus work: 670770b9-8ded-5643-844c-1c6d5b62687c
DOI: 10.64898/2025.12.24.696356

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