Article

Microhomology-mediated CRISPR/Cas9-based method for genome editing in fission yeast

2018-12-17

Abstract excerpt

The CRISPR/Cas9 system enables the editing of genomes of numerous organisms through the induction of the double-strand breaks (DSB) at specific chromosomal targets. We improved the CRISPR/Cas9 system to ease the direct introduction of a point mutation or a tagging sequence into the chromosome by combining it with the microhomology mediated end joining (MMEJ)-based genome editing in fission yeast. We constructed co...

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Literature Corpus work: c3f5b091-6f07-5397-bd65-8e7b3ebea912
DOI: 10.1101/498402

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