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Article

CRISPR/Cas9 - Mediated Precise Targeted Integration In Vivo Using a Double Cut Donor with Short Homology Arms.

EBioMedicine - 1 Jun 2017

Yao Xuan, Wang Xing, Liu Junlai, Hu Xinde, Shi Linyu, Shen Xiaowen, Ying Wenqin, Sun Xinyao, Wang Xin, Huang Pengyu, Yang Hui

Abstract excerpt

Precisely targeted genome editing is highly desired for clinical applications. However, the widely used homology-directed repair (HDR)-based genome editing strategies remain inefficient for certain in vivo applications. We here demonstrate a microhomology-mediated end-joining (MMEJ)-based strategy for precisely targeted gene integration in transfected neurons and hepatocytes in vivo with efficiencies up to 20%,...

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Source

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Identifiers

PMID: 28527830
DOI: 10.1016/j.ebiom.2017.05.015
PMCID: PMC5478232

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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