Article

Nanopore sequencing of native adeno-associated virus (AAV) single-stranded DNA using a transposase-based rapid protocol

2019-12-28

Abstract excerpt

<h4>ABSTRACT</h4> Next-generation sequencing of single-stranded DNA (ssDNA) enables transgene characterization of gene-therapy vectors such as adeno-associated virus (AAV), but current library generation uses complicated and potentially biased second-strand synthesis. We report that libraries for nanopore sequencing of ssDNA can be conveniently created without second-strand synthesis using a transposase-based pro...

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Identifiers and source

Literature Corpus work: bec85543-7310-5bef-8efc-1a65ec850028
DOI: 10.1101/2019.12.27.885319

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