Article

High-Resolution Characterization of rAAV Genomes for Vector Quality Using Long-Read Sequencing

2025-10-06

Abstract excerpt

Recombinant adeno-associated virus (rAAV)-mediated gene therapy has been applied for human diseases. However, the rAAV capsids contain heterogeneous mixtures of full-length and truncated genomes and, depending on the manufacturing process, residual host cell and plasmid DNA. Therefore, a method is needed to characterize the encapsidated DNA of rAAV in order to support process development and batch release. The eme...

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Literature Corpus work: 82d57087-80e6-5139-8de2-46f7846fefee
DOI: 10.1101/2025.10.05.680573

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