Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Accurate Identification and Quantification of DNA Species by Next-Generation Sequencing in Adeno-Associated Viral Vectors Produced in Insect Cells

2 May 2017

Abstract excerpt

Recombinant adeno-associated viral (rAAV) vectors have proven excellent tools for the treatment of many genetic diseases and other complex diseases. However, the illegitimate encapsidation of DNA contaminants within viral particles constitutes a major safety concern for rAAV-based therapies. Moreover, the development of rAAV vectors for early-phase clinical trials has revealed the limited accuracy of the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CAR-T cell therapy research
Share to Viral Infectious Diseases and Gene Expression in Insects
Share to Virus-based gene therapy research

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 28463571
DOI: 10.1089/hgtb.2016.185

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Accurate Identification and Quantification of DNA Species by Next-Generation Sequencing in Adeno-Associated Viral Vectors Produced in Insect CellsPMID 28463571Read ↗

High-Resolution Characterization of rAAV Genomes for Vector Quality Using Long-Read SequencingSemantic proximity Read ↗

Residual DNA impurities in AAV vectors—nature and transcriptionSemantic proximity Read ↗

Native Molecular State of Adeno-Associated Viral Vectors Revealed by Single-Molecule SequencingSemantic proximity Read ↗

Stowaways in the cargo: Contaminating nucleic acids in rAAV preparations for gene therapySemantic proximity Read ↗

Precision DNA Impurity Reduction Approaches for Ultra-Pure rAAV ManufacturingSemantic proximity Read ↗

A Universal Next-Generation Sequencing Protocol To Generate Noninfectious Barcoded cDNA Libraries from High-Containment RNA VirusesSemantic proximity Read ↗

Comparative next-generation sequencing of adeno-associated virus inverted terminal repeats.Semantic proximity Read ↗

Development, applications, and future prospects of RNA virus reverse genetics technologySemantic proximity Read ↗

Methods Matter -- Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct VectorsSemantic proximity Read ↗

Applying next-generation sequencing to unravel the mutational landscape in viral quasispecies.Semantic proximity Read ↗

Next generation sequencing of viral RNA genomes.Semantic proximity Read ↗

High-sensitivity whole-genome recovery of single viral species in environmental samplesSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.