Article
Direct RNA nanopore sequencing of full-length coronavirus genomes provides novel insights into structural variants and enables modification analysis.
Genome research - 1 Sept 2019
Viehweger Adrian, Krautwurst Sebastian, Lamkiewicz Kevin, Madhugiri Ramakanth, Ziebuhr John, Hölzer Martin, Marz Manja
Abstract excerpt
Sequence analyses of RNA virus genomes remain challenging owing to the exceptional genetic plasticity of these viruses. Because of high mutation and recombination rates, genome replication by viral RNA-dependent RNA polymerases leads to populations of closely related viruses, so-called "quasispecies." Standard (short-read) sequencing technologies are ill-suited to reconstruct large numbers of full-length...
Read the complete abstract on PubMedTopics
- Cell Line
- Coronavirus
- Evolution, Molecular
- Genetic Variation
- Genome Size
- Humans