Article
Structure and dynamics of a primordial catalytic fold generated by in vitro evolution.
Nature chemical biology - 1 Feb 2013
Chao Fa-An, Morelli Aleardo, Haugner John C, Churchfield Lewis, Hagmann Leonardo N, Shi Lei, Masterson Larry R, Sarangi Ritimukta, Veglia Gianluigi, Seelig Burckhard
Abstract excerpt
Engineering functional protein scaffolds capable of carrying out chemical catalysis is a major challenge in enzyme design. Starting from a noncatalytic protein scaffold, we recently generated a new RNA ligase by in vitro directed evolution. This artificial enzyme lost its original fold and adopted an entirely new structure with substantially enhanced conformational dynamics, demonstrating that a primordial fold...
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