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Article

Visualization and genetic analysis of alternative splicing regulation in vivo using fluorescence reporters in transgenic Caenorhabditis elegans.

Nature protocols - 1 Sept 2010

Kuroyanagi Hidehito, Ohno Genta, Sakane Hiroaki, Maruoka Hiroyuki, Hagiwara Masatoshi

Abstract excerpt

Transgenic multicolor fluorescence reporters enable the visualization of alternative splicing patterns at a single-cell resolution in living organisms and facilitate further genetic analyses to identify cis-elements and trans-acting factors involved in splicing regulation. In this paper, we describe a method of generating fluorescence alternative splicing reporters for the nematode Caenorhabditis elegans. We...

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PMID: 20725066
DOI: 10.1038/nprot.2010.107

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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