Article

Fluorescent Reporters Give New Insights Into Antibiotics-induced Mistranslation

2023-11-30

Abstract excerpt

We developed a reporter system based on simultaneous expression of two fluorescent proteins: GFP as a reporter of the capacity of protein synthesis and mutated mScarlet-I as a reporter of translational errors. Because of the unique stop codons or frameshift mutations introduced into the mScarlet-I gene, red fluorescence was produced only after a mistranslation event. These reporters allowed us to estimate mistrans...

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Literature Corpus work: b83c756a-66bf-5ae6-8b1c-bab972765929
DOI: 10.21203/rs.3.rs-3615791/v1

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