Article
Visualizing high error levels during gene expression in living bacterial cells.
Proceedings of the National Academy of Sciences of the United States of America - 22 Jun 2010
Meyerovich Mor, Mamou Gideon, Ben-Yehuda Sigal
Abstract excerpt
To monitor inaccuracy in gene expression in living cells, we designed an experimental system in the bacterium Bacillus subtilis whereby spontaneous errors can be visualized and quantified at a single-cell level. Our strategy was to introduce mutations into a chromosomally encoded gfp allele, such that errors in protein production are reported in real time by the formation of fluorescent GFP molecules. The data...
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