Article

Unbiased sequencing of <i>Mycobacterium tuberculosis</i> urinary cell-free DNA reveals extremely short fragment lengths

2021-06-25

Abstract excerpt

<h4>ABSTRACT</h4> Urine cell-free DNA (cfDNA) presents an attractive target for diagnosing pulmonary Mycobacterium tuberculosis (TB) infection but has not been thoroughly characterized. Here, we aimed to investigate the size and composition of TB-derived urine cfDNA with minimal bias using next-generation DNA sequencing (NGS). To enable analysis of highly fragmented urine cfDNA, we used a combination of DNA extrac...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: b29b1b84-47d3-55f3-ab8c-6efb5463f8bd
DOI: 10.1101/2021.06.22.21258723

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Unbiased sequencing of <i>Mycobacterium tuberculosis</i> urinary cell-free DNA reveals extremely short fragment lengthsDOI 10.1101/2021.06.22.21258723Read

Characterizing the molecular composition and diagnostic potential of Mycobacterium tuberculosis urinary cell-free DNA using next-generation sequencingSemantic proximity Read

Structure and diversity of urinary cell-free DNA informative of host-pathogen interactions in human urinary tract infectionSemantic proximity Read

Urinary cell-free DNA is a versatile analyte for monitoring infections of the urinary tractSemantic proximity Read

Protocol for DNA Extraction from QuantiFERON-TB Gold Tubes for PCR and Sequencing ApplicationsSemantic proximity Read

Host DNA depletion assisted metagenomic sequencing of bronchoalveolar lavage fluids for diagnosis of pulmonary tuberculosisSemantic proximity Read

Single-stranded DNA library preparation uncovers the origin and diversity of ultrashort cell-free DNA in plasmaSemantic proximity Read

Analytical Validation of a Highly Accurate and Reliable Next-Generation Sequencing-Based Urine AssaySemantic proximity Read

Direct nanopore sequencing of <i>M. tuberculosis</i> on sputa and rescue of suboptimal results to enhance transmission surveillanceSemantic proximity Read

A needle in a haystack: metagenomic DNA sequencing to quantify <i>Mycobacterium tuberculosis</i> DNA and diagnose tuberculosisSemantic proximity Read

Sub-nucleosomal organization in urine cell-free DNASemantic proximity Read

Direct capture and sequencing reveal ultra-short single-stranded DNA in biofluidsSemantic proximity Read

A scalable, efficient, and safe method to prepare high quality DNA from mycobacteria and other challenging cellsSemantic proximity Read