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Interactive computational and experimental approaches improve the sensitivity of periplasmic binding protein-based nicotine biosensors for measurements in biofluids

2023-01-19

Abstract excerpt

We developed fluorescent protein sensors for nicotine with improved sensitivity. For iNicSnFR12 at pH 7.4, the proportionality constant for ΔF/F 0 vs [nicotine] (δ-slope, 2.7 μM -1 ) is 6.1-fold higher than the previously reported iNicSnFR3a. The activated state of iNicSnFR12 has a fluorescence quantum yield of at least 0.6. We measured similar dose-response relations for the nicotine-induced absorbance increase...

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Literature Corpus work: af5c94a0-4922-5d57-963e-c0b41edd0ebf
DOI: 10.1101/2023.01.16.524298

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