Article
Three Mutations Convert the Selectivity of a Protein Sensor from Nicotinic Agonists to S-Methadone for Use in Cells, Organelles, and Biofluids.
Journal of the American Chemical Society - 18 May 2022
Muthusamy Anand K, Kim Charlene H, Virgil Scott C, Knox Hailey J, Marvin Jonathan S, Nichols Aaron L, Cohen Bruce N, Dougherty Dennis A, Looger Loren L, Lester Henry A
Abstract excerpt
We report a reagentless, intensity-based S-methadone fluorescent sensor, iS-methadoneSnFR, consisting of a circularly permuted GFP inserted within the sequence of a mutated bacterial periplasmic binding protein (PBP). We evolved a previously reported nicotine-binding PBP to become a selective S-methadone-binding sensor, via three mutations in the PBP's second shell and hinge regions. iS-methadoneSnFR displays the...
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