Article

Three Mutations Convert the Selectivity of a Protein Sensor from Nicotinic Agonists to S-methadone for Use in Cells, Organelles, and Biofluids

2022-02-26

Abstract excerpt

<h4>ABSTRACT</h4> We report a reagentless, intensity-based S-methadone fluorescent sensor, iS-methadoneSnFR, consisting of a circularly permuted GFP inserted within the sequence of a mutated bacterial periplasmic binding protein (PBP). We evolved a previously reported nicotine-binding PBP to become a selective S-methadone-binding sensor, via three mutations in the PBP’s second shell and hinge regions. iS-methadon...

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Literature Corpus work: e4f86087-098d-55c1-95b7-3d5d0f98e6a0
DOI: 10.1101/2022.02.24.481226

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