Expansion of the Prime editing Modality with Cas9 from Francisella novicida

Abstract excerpt

<title>Abstract</title> <p>Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase (RT) after nick formation by CRISPR nickase. In this study, we developed a technology that can be used to insert or replace external bases in the target DNA sequence by linking reverse transcriptase to the Francisella novicida Cas9, which is a CRISPR-Cas9 ortholog. U...

Topics

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• CRISPR-Cas Systems
• Gene Editing
• Genetic Diseases, Inborn
• Genetic Therapy
• INDEL Mutation
• Mutation
• Neoplasms
• RNA-Directed DNA Polymerase

Identifiers and source

Literature Corpus work

ad626696-7c2a-591b-a6dc-20531efb022a

DOI

10.21203/rs.3.rs-628506/v1