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Use antibiotics in cell culture with caution: genome-wide identification of antibiotic-induced changes in gene expression and regulation

2017-04-25

Abstract excerpt

Standard cell culture guidelines often use media supplemented with antibiotics to prevent cell contamination. However, relatively little is known about the effect of antibiotic use in cell culture on gene expression and the extent to which this treatment could confound results. To comprehensively characterize the effect of antibiotic treatment on gene expression, we performed RNA-seq and ChIP-seq for H3K27ac on He...

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Literature Corpus work
ad569301-c1b9-5a7d-8a5c-297810d8685a
DOI
10.1101/130484
Open publication

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Use antibiotics in cell culture with caution: genome-wide identification of antibiotic-induced changes in gene expression and regulationDOI 10.1101/130484
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