Article

Cell line identity rather than medium composition determines transcriptomic profiles of HepaRG and HuH7 cells cultured in chemically defined or serum-based media: comparison with primary human hepatocytes

2026-03-11

Abstract excerpt

The composition of culture medium is a major, yet frequently undercontrolled, determinant of hepatic cell state in vitro . For decades, fetal bovine serum (FBS) has been routinely incorporated into liver cell culture. Its undefined and lot-to-lot variable composition can, however, confound cell identity and experimental reproducibility. Serum-free, chemically defined media (CDM) represent an alternative approach...

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Literature Corpus work: bc686637-a434-5fba-8925-3b419816deb9
DOI: 10.64898/2026.03.09.710463

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