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A Novel Multiplex qPCR Platform for Detection of <i>Mycobacterium tuberculosis</i> Drug Resistance Using Synthetic Mutation Standards and Fluorescence Quenching Probes

2025-06-11

Abstract excerpt

The emergence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium tuberculosis strains has intensified the need for rapid, accurate, and affordable molecular diagnostics. In this study, we report the development and analytical validation of a multiplex real-time PCR assay employing contact-quenching fluorescence detection, allele-specific primers, and 3′ blocked wild-type allele blocke...

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Literature Corpus work
aae555c4-4559-5742-aa18-deb63de21f4a
DOI
10.1101/2025.06.10.25329335
Open publication

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A Novel Multiplex qPCR Platform for Detection of <i>Mycobacterium tuberculosis</i> Drug Resistance Using Synthetic Mutation Standards and Fluorescence Quenching ProbesDOI 10.1101/2025.06.10.25329335
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