Article
Detection of rifampin resistance in Mycobacterium tuberculosis in a single tube with molecular beacons.
Journal of clinical microbiology - 1 Nov 2001
El-Hajj H H, Marras S A, Tyagi S, Kramer F R, Alland D
Abstract excerpt
Current clinical assays for determining antibiotic susceptibility in Mycobacterium tuberculosis require many weeks to complete due to the slow growth of the bacilli. Here we demonstrate an extremely sensitive single-tube PCR assay that takes less than 3 h and reliably identifies rifampin-resistant M. tuberculosis in DNA extracted directly from sputum. Ninety-five percent of mutations associated with rifampin...
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