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Ambient-Temperature Extraction-Free One-Pot CRISPR Detection of <i>Mycobacterium tuberculosis</i>

2026-07-22

Abstract excerpt

<h4>ABSTRACT</h4> <h4>Background</h4> Current molecular diagnostics for Mycobacterium tuberculosis (MTB) require complex nucleic acid extraction procedures and laboratory infrastructure, limiting their use as point-of-care (POC) tests in resource-limited settings. To address these barriers, we developed an extraction-free one-pot CRISPR assay for rapid detection of MTB directly from minimally processed sputum s...

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Literature Corpus work
00d09f49-5f00-56e2-9d5d-d3472493f53c
DOI
10.64898/2026.07.21.26358604
Open publication

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