Article

Tuning Mobile Phase Properties to Improve Empty Full Particle Separation in Adeno-associated Virus by Anion Exchange Chromatography

2023-02-09

Abstract excerpt

In the past decade, recombinant adeno-associated virus (rAAV) has gained increased attention as a prominent gene therapy technology to treat monogenetic disease. One of the challenges in rAAV production is the enrichment of full-rAAV particles containing the gene of interested (GOI) payload. Herein, we demonstrated that by adjusting the mobile phase properties of anion-exchange chromatography (AEX), Empty and Full...

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Literature Corpus work: a9873197-f133-523a-a642-f5d7a9b3b6b3
DOI: 10.22541/au.167592246.61877510/v1

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