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Arrayed CRISPRi and Quantitative Imaging Describe the Morphotypic Landscape of Essential Mycobacterial Genes

2020-03-22

Abstract excerpt

<h4>ABSTRACT</h4> Mycobacterium tuberculosis possesses a large number of genes of unknown or merely predicted function, undermining fundamental understanding of pathogenicity and drug susceptibility. To address this challenge, we developed a high-throughput functional genomics approach combining inducible CRISPR-interference and image-based analyses of morphological features and sub-cellular molecular localizatio...

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Literature Corpus work
a70de82c-8390-59e8-80ad-82bce1abc4f6
DOI
10.1101/2020.03.20.000372
Open publication

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Arrayed CRISPRi and Quantitative Imaging Describe the Morphotypic Landscape of Essential Mycobacterial GenesDOI 10.1101/2020.03.20.000372
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