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Arrayed CRISPRi and quantitative imaging describe the morphotypic landscape of essential mycobacterial genes

5 Nov 2020

Abstract excerpt

Mycobacterium tuberculosis possesses a large number of genes of unknown or predicted function, undermining fundamental understanding of pathogenicity and drug susceptibility. To address this challenge, we developed a high-throughput functional genomics approach combining inducible CRISPR-interference and image-based analyses of morphological features and sub-cellular chromosomal localizations in the related...

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PMID: 33155979
DOI: 10.7554/elife.60083

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