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Article

Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries.

Scientific reports - 22 May 2017

Metzakopian Emmanouil, Strong Alex, Iyer Vivek, Hodgkins Alex, Tzelepis Konstantinos, Antunes Liliana, Friedrich Mathias J, Kang Qiaohua, Davidson Teresa, Lamberth Jacob, Hoffmann Christina, Davis Gregory D, Vassiliou George S, Skarnes William C, Bradley Allan

Abstract excerpt

CRISPR-Cas9 technology has accelerated biological research becoming routine for many laboratories. It is rapidly replacing conventional gene editing techniques and has high utility for both genome-wide and gene-focussed applications. Here we present the first individually cloned CRISPR-Cas9 genome wide arrayed sgRNA libraries covering 17,166 human and 20,430 mouse genes at a complexity of 34,332 sgRNAs for human...

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PMID: 28533524
DOI: 10.1038/s41598-017-01766-5
PMCID: PMC5440395

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