Article

An efficient method for immortalizing mouse embryonic fibroblasts

2024-06-30

Abstract excerpt

Mouse embryonic fibroblasts (MEFs) derived from genetically modified mice are a valuable resource for studying gene function and regulation. The MEF system can also be combined with rescue studies to characterize the function of mutant genes/proteins, such as disease-causing variants. However, primary MEFs undergo senescence soon after isolation and passaging, making long-term genetic manipulations difficult. Prev...

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Literature Corpus work: a24d5a9c-6e41-545f-a19e-4c8a73dc7cb3
DOI: 10.1101/2024.06.27.601088

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