Article
One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs.
Cell research - 1 Jul 2017
Zuo Erwei, Cai Yi-Jun, Li Kui, Wei Yu, Wang Bang-An, Sun Yidi, Liu Zhen, Liu Jiwei, Hu Xinde, Wei Wei, Huo Xiaona, Shi Linyu, Tang Cheng, Liang Dan, Wang Yan, Nie Yan-Hong, Zhang Chen-Chen, Yao Xuan, Wang Xing, Zhou Changyang, Ying Wenqin, Wang Qifang, Chen Ren-Chao, Shen Qi, Xu Guo-Liang, Li Jinsong, Sun Qiang, Xiong Zhi-Qi, Yang Hui
Abstract excerpt
The CRISPR/Cas9 system is an efficient gene-editing method, but the majority of gene-edited animals showed mosaicism, with editing occurring only in a portion of cells. Here we show that single gene or multiple genes can be completely knocked out in mouse and monkey embryos by zygotic injection of Cas9 mRNA and multiple adjacent single-guide RNAs (spaced 10-200 bp apart) that target only a single key exon of each...
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