Article

Immortalization of Human Primary CD8<sup>+</sup> T cells by Inserting a Single Copy of Human Telomerase Reverse Transcriptase via CRISPR/Cas9

2024-08-29

Abstract excerpt

<h4>Background: </h4> Existing human cell immortalization methods made the cells obtain oncogenesis phenotype and/or caused the cells gain and/or lose chromosomes. Immortalized normal human T cells lines provide critical in vitro models for basic research and therapeutic products development. <h4>Methods:</h4> We utilized a CRISPR/Cas9 system to replace a single copy of the exon 2 of the cell cycle inhibitor gene...

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Literature Corpus work: 2915242b-f461-5bb6-bc93-65bea8b4274a
DOI: 10.20944/preprints202408.2129.v1

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