Back to search

Article

Non-Destructive Larval Genotyping of <i>Danio rerio</i> for Mitochondrial and Nuclear DNA Genetics

2025-05-01

Abstract excerpt

The rapid advancement of nuclear and mitochondrial genomic editing tools has created an urgent need for efficient, non-lethal larval genotyping methods in zebrafish ( Danio rerio ) research. This study optimizes and validates a non-destructive proteinase K digestion method for mitochondrial and nuclear DNA genotyping while characterizing its impact on larval survival and gene expression. Using optimized protocol...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
9f417a9e-ddbc-50a8-add3-27a2db5f03a1
DOI
10.1101/2025.04.30.651585
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Non-Destructive Larval Genotyping of <i>Danio rerio</i> for Mitochondrial and Nuclear DNA GeneticsDOI 10.1101/2025.04.30.651585
A Primer Genetic Toolkit for Exploring Mitochondrial Biology and Disease Using ZebrafishSemantic proximity
A primer genetic toolkit for exploring mitochondrial biology and disease using zebrafishSemantic proximity
Automated multi-sample DNA extraction for genotyping live Xenopus embryos.Semantic proximity
Mitochondrial genome diversity among six laboratory zebrafish (Danio rerio) strains.Semantic proximity
Engineering targeted deletions in the mitochondrial genomeSemantic proximity
The FusX TALE Base Editor (FusXTBE) for rapid mitochondrial DNA programming of human cells <i>in vitro</i> and zebrafish disease models <i>in vivo</i>Semantic proximity
Precise modelling of mitochondrial diseases using optimized mitoBEs.Semantic proximity
Mitochondrial genome editing tools: prospects in animal breeding.Semantic proximity
High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping.Semantic proximity
Delivery of mtZFNs into Early Mouse Embryos.Semantic proximity
A large-scale zebrafish gene knockout resource for the genome-wide study of gene function.Semantic proximity
In vivo genome editing using a high-efficiency TALEN system.Semantic proximity
Select a neighboring publication to make it the new centre.