Article
Nondestructive Larval Genotyping of Danio rerio for Mitochondrial and Nuclear DNA Genetics.
Zebrafish - 1 Dec 2025
Mitra Kyler S, Holmberg Shannon R, Mota Mireya, Sabharwal Ankit, Ekker Stephen C
Abstract excerpt
The rapid advancement of nuclear and mitochondrial genomic editing tools has created an urgent need for efficient, nonlethal larval genotyping methods in zebrafish (Danio rerio) research. This study optimizes and validates a nondestructive proteinase K digestion method for mitochondrial and nuclear DNA genotyping while characterizing its impact on larval survival and gene expression. Using optimized protocol...
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- Animals
- Zebrafish
- Larva
- Genotyping Techniques
- DNA, Mitochondrial
- Genotype
- Cell Nucleus