Article

ediTONSIL: Activation-Neutral Gene Editing of CD4 T cells in Human ex Vivo Tonsil Cultures

2023-08-22

Abstract excerpt

<h4>Motivation</h4> CD4 T cells are central players of adaptive immunity that rapidly change activation states in response to exogenous T cell receptor (TCR) stimulation. While molecular processes in activated human CD4 T cells from peripheral blood are well studied, resting CD4 T cells are refractory to gene-editing transduction and transfection methods without prior activation. Knowledge on the molecular biolog...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 9dc044eb-118c-5b1d-85b4-7e9963c1d2f5
DOI: 10.1101/2023.08.21.554089

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

ediTONSIL: Activation-Neutral Gene Editing of CD4 T cells in Human ex Vivo Tonsil CulturesDOI 10.1101/2023.08.21.554089Read

Rapid, efficient and activation-neutral gene editing of polyclonal primary human resting CD4+ T cells allows complex functional analysesSemantic proximity Read

Rapid and efficient generation of antigen-specific isogenic T cells from cryopreserved blood samplesSemantic proximity Read

Protocol for Non-viral HDR-based CRISPR/Cas9 platform for small custom editing in primary T cellsSemantic proximity Read

A CRISPR-Cas9 Genome Engineering Platform in Primary CD4+ T Cells for the Interrogation of HIV Host FactorsSemantic proximity Read

Generation of knock-in primary human T cells using Cas9 ribonucleoproteinsSemantic proximity Read

A method for polyclonal antigen-specific T cell-targeted genome editing (TarGET) for adoptive cell therapy applicationsSemantic proximity Read

Unraveling the Phenotypic States of Human innate-like T Cells: Comparative Insights with Conventional T Cells and Mouse ModelsSemantic proximity Read

Ribonucleoprotein Transfection for CRISPR/Cas9‐Mediated Gene Knockout in Primary T CellsSemantic proximity Read

Generating universal anti-CD19 CAR T cells with a defined memory phenotype by CRISPR/Cas9 editing and safety evaluation of the transcriptome.Semantic proximity Read

Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune FunctionSemantic proximity Read

Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome EngineeringSemantic proximity Read

The clinical potential of gene editing as a tool to engineer cell‐based therapeuticsSemantic proximity Read