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Article

Generation of knock-in primary human T cells using Cas9 ribonucleoproteins

27 Jul 2015

Abstract excerpt

T-cell genome engineering holds great promise for cell-based therapies for cancer, HIV, primary immune deficiencies, and autoimmune diseases, but genetic manipulation of human T cells has been challenging. Improved tools are needed to efficiently "knock out" genes and "knock in" targeted genome modifications to modulate T-cell function and correct disease-associated mutations. CRISPR/Cas9 technology is...

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PMID: 26216948
DOI: 10.1073/pnas.1512503112

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