Article

Rapid and efficient generation of antigen-specific isogenic T cells from cryopreserved blood samples

2021-11-13

Abstract excerpt

<h4>ABSTRACT</h4> <h4>Objectives</h4> CRISPR/Cas9-mediated gene editing has been leveraged for the modification of human and mouse T cells. However, limited experience is available on the application of CRISPR/Cas9 electroporation in cryopreserved T cells collected during e.g. clinical trials. <h4>Methods</h4> PBMCs from healthy donors were used to generate knockout T cell models for interferon-γ (IFNγ), Cbl Pr...

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Literature Corpus work: 5511e2db-bc62-5c14-99ff-7ca329115ab8
DOI: 10.1101/2021.11.12.468355

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