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Article

Ribonucleoprotein Transfection for CRISPR/Cas9‐Mediated Gene Knockout in Primary T Cells

18 Oct 2018

Abstract excerpt

CRISPR/Cas9 has enabled the rapid and efficient generation of gene knockouts across various cell types of several species. T cells are central players in adaptive immune responses. Gene editing in primary T cells not only represents a valuable research tool, but is also critical for next generation immunotherapies, such as CAR T cells. Broad application of CRIPSR/Cas9 for gene editing in primary T cells has been...

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PMID: 30334617
DOI: 10.1002/cpim.69

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