Article

Assessment of interkingdom TIR domain interactions using a modular yeast tripartite split-GFP system

2026-03-25

Abstract excerpt

<title>Abstract</title> <p> Thanks to its genetic resourcefulness, <italic>Saccharomyces cerevisiae</italic> has been traditionally used as a model organism to detect and study protein-protein interactions. The recent development of synthetic biology toolkits brings an opportunity to expand such utilities. Here, we coupled the modular cloning (MoClo) system to the tripartite split-GFP strategy to systematicall...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 99fdf1b0-6d2c-57cc-b89d-ece1e689d0ab
DOI: 10.21203/rs.3.rs-9032499/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Assessment of interkingdom TIR domain interactions using a modular yeast tripartite split-GFP systemDOI 10.21203/rs.3.rs-9032499/v1Read

Trypanosoma brucei RAP1 Has Essential Functional Domains That Are Required for Different Protein Interactions.Semantic proximity Read

New Gateway-compatible vectors for a high-throughput protein-protein interaction analysis by a bimolecular fluorescence complementation (BiFC) assay in plants and their application to a plant clathrin structure analysis.Semantic proximity Read

Detection of Dual Targeting and Dual Function of Mitochondrial Proteins in Yeast.Semantic proximity Read

Ypt1 and TRAPP interactions: optimization of multicolor bimolecular fluorescence complementation in yeast.Semantic proximity Read

Reconstructing the TIR Side of the Myddosome: a Paradigm for TIR-TIR Interactions.Semantic proximity Read

Proteome survey reveals modularity of the yeast cell machinery.Semantic proximity Read

Structural and functional studies of the first tripartite protein complex at the <i>Trypanosoma brucei</i> flagellar pocket collarSemantic proximity Read

Pooled PPIseq: screening the SARS-CoV-2 and human interface with a scalable multiplexed protein-protein interaction assay platformSemantic proximity Read

Independent evolution of functionally exchangeable mitochondrial outer membrane import complexesSemantic proximity Read

A new protein-protein interaction sensor based on tripartite split-GFP association.Semantic proximity Read

A single-cysteine mutant and chimeras of essential Leishmania Erv can complement the loss of Erv1 but not of Mia40 in yeast.Semantic proximity Read

Protein-protein interactions: the yeast two-hybrid system.Semantic proximity Read